mig-10 : unc-6

In unc-6(ev400) single mutants, all ACs attached to the BM at the P6.p 1-cell stage prior to the invasion. The ACs later started to detach from the BM staying afloat in the gonad. The percentage of detached ACs increased to 10% and 15% at the P6.p 2- and 4-cell stages, respectively, suggesting that UNC-6 promotes AC-BM adhesion during AC invasion. In contrast, mig-10(ct41) mutants had no phenotypic defects in AC-BM attachment. However, when mig-10 was removed in unc-6 mutants, AC detachment was observed at the P6.p 1-cell stage, revealing an early requirement for MIG-10B in 215 stabilizing AC-BM attachment . AC detachment was later drastically increased by approximately 4-6 folds at the P6.p 2- and 4-cell stages, indicating a synergistic effect between MIG-10B and UNC-6 on mediating AC-BM adhesion during AC invasion.

hic-1 : nab-1

"HIC-1 interacts with NAB-1 through its PDZ(bm) and that NAB-1 localization is dependent on HIC-1 expression."

emb-9 : fbl-1

EMB-9 is secreted from the body wall muscle cells and localizes to the BM. An antibody against EMB-9 clearly stained gonadal and intestinal BMs in wild-type hermaphrodites at the third and fourth larval stages (L3 and L4, respectively). EMB-9 accumulation in fbl-1(tk45) mutants was normal at L3 but was markedly reduced and punctate at L4. These results suggest that FBL-1 is not required for gonadal accumulation of EMB-9 prior to L4, but for maintaining EMB-9 localization in the gonadal BM during the late larval stages.