pam-1 : par-2

In wild-type one-cell embryos, PAR-2 localized to the cortex in the posterior. PAR-2 was not detected at the cortex in 44.5% of pam-1 mutant embryos. In 60% of embryos with detectable PAR-2 at the cortex, it was mislocalized to a small lateral patch, which did not correlate with meiotic spindle position or polar body location. In about 56% of pam-1 mutant embryos, PAR-2 was detected in puncta near the centrosomes and their asters. While this peri-centrosome staining occurred most frequently in embryos lacking cortical PAR-2, it was also observed in embryos in which cortical PAR-2 was mislocalized or properly localized. Only 20% of one-cell pam-1 mutant embryos showed normal cortical PAR-2 staining restricted to the posterior pole.

pam-1 : par-2

In wild-type one-cell embryos, PAR-2 localized to the cortex in the posterior. PAR-2 was not detected at the cortex in 44.5% of pam-1 mutant embryos. In 60% of embryos with detectable PAR-2 at the cortex, it was mislocalized to a small lateral patch, which did not correlate with meiotic spindle position or polar body location. In about 56% of pam-1 mutant embryos, PAR-2 was detected in puncta near the centrosomes and their asters. While this peri-centrosome staining occurred most frequently in embryos lacking cortical PAR-2, it was also observed in embryos in which cortical PAR-2 was mislocalized or properly localized. Only 20% of one-cell pam-1 mutant embryos showed normal cortical PAR-2 staining restricted to the posterior pole.