11 Interactions found (0.009 seconds)

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... We also observed prominent GFP expression in the AIM interneurons (Figure 4A). To distinguish the AIM from the neighboring AIY neuron, we crossed pCRE::GFP worms with pAIY::RFP (Wenick and Hobert, 2004) and found that GFP and RFP do not overlap in the bigenic animals after training (Figure 4A), confirming CREB activity in the AIM. The activation of CREB in the AIM is specific to memory training, since GFP expression is not detected in the AIM upon mock training (Figure 4B)."

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... Many CREB/LTAM-induced genes that are required for longterm memory (Figure 3) are not detected in AIM neurons (Figure S7). Rather, their expression is prominent in neurons of the nerve ring, or in socket cell glia in the case of F21G4.1, or both (ins-22). Knockdown of F21G4.1 blocks 16 hr memory (Figure 3G), suggesting that socket cell glia may play a role in LTAM. The PQN-73 prion-like protein is expressed in the arcade cells and is also required for LTAM (Figures S7 and 3G)."

crh-1 : jnk-1 : long-term memory training

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... pCRE::GFP expression in the SIA, where CREB is activated upon extended starvation (Suo et al., 2006) (Figure 4A), was expected, since our training paradigm involves cycling between starvation and food paired with butanone. Fluorescence in the SIA neurons was also observed upon mock training in the absence of butanone (Figure 4B). However, CREB rescue in the SIA neurons does not rescue LTAM (Figure 4E), suggesting that CREB activation in the SIA is not specific to or required for long-term memory."

ins-22 : long-term memory training

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... Many CREB/LTAM-induced genes that are required for longterm memory (Figure 3) are not detected in AIM neurons (Figure S7). Rather, their expression is prominent in neurons of the nerve ring, or in socket cell glia in the case of F21G4.1, or both (ins-22). Knockdown of F21G4.1 blocks 16 hr memory (Figure 3G), suggesting that socket cell glia may play a role in LTAM. The PQN-73 prion-like protein is expressed in the arcade cells and is also required for LTAM (Figures S7 and 3G)."

ins-22 : long-term memory training

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... Many CREB/LTAM-induced genes that are required for longterm memory (Figure 3) are not detected in AIM neurons (Figure S7). Rather, their expression is prominent in neurons of the nerve ring, or in socket cell glia in the case of F21G4.1, or both (ins-22). Knockdown of F21G4.1 blocks 16 hr memory (Figure 3G), suggesting that socket cell glia may play a role in LTAM. The PQN-73 prion-like protein is expressed in the arcade cells and is also required for LTAM (Figures S7 and 3G)."

long-term memory training : unc-43

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... Many CREB/LTAM-induced genes that are required for longterm memory (Figure 3) are not detected in AIM neurons (Figure S7). Rather, their expression is prominent in neurons of the nerve ring, or in socket cell glia in the case of F21G4.1, or both (ins-22). Knockdown of F21G4.1 blocks 16 hr memory (Figure 3G), suggesting that socket cell glia may play a role in LTAM. The PQN-73 prion-like protein is expressed in the arcade cells and is also required for LTAM (Figures S7 and 3G)."

ccdc-149 : long-term memory training

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... Many CREB/LTAM-induced genes that are required for longterm memory (Figure 3) are not detected in AIM neurons (Figure S7). Rather, their expression is prominent in neurons of the nerve ring, or in socket cell glia in the case of F21G4.1, or both (ins-22). Knockdown of F21G4.1 blocks 16 hr memory (Figure 3G), suggesting that socket cell glia may play a role in LTAM. The PQN-73 prion-like protein is expressed in the arcade cells and is also required for LTAM (Figures S7 and 3G)."

long-term memory training : unc-44

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... Many CREB/LTAM-induced genes that are required for longterm memory (Figure 3) are not detected in AIM neurons (Figure S7). Rather, their expression is prominent in neurons of the nerve ring, or in socket cell glia in the case of F21G4.1, or both (ins-22). Knockdown of F21G4.1 blocks 16 hr memory (Figure 3G), suggesting that socket cell glia may play a role in LTAM. The PQN-73 prion-like protein is expressed in the arcade cells and is also required for LTAM (Figures S7 and 3G)."

crh-1 : jnk-1

"we used reporter transgenic worms in which GFP expression is controlled by the CRE reporter (pCRE::GFP) (Kimura et al., 2002). In untrained worms, CREB activity is barely detectible (Figure 4A). After spaced butanone/food long-term memory training, we observed fluorescence in two sets of neurons (Figures 4A and 4B)... To examine the dynamics of CREB activation, we quantified the levels of pCRE::GFP fluorescence in populations of LTAM-trained worms. While phosphorylated CREB levels rise fairly linearly with training, pCRE::GFP fluorescence increased markedly after 3-4 training sessions, paralleling the increase in LTAM performance (Figures 5A and 5D). Mutants of unc-43 and jnk-1, both activators of CREB (Johannessen et al., 2004) and downstream targets of CREB (Table S5), blocked the pCRE::GFP fluorescence increase (Figures 5B-5D), suggesting that a feedforward mechanism of CREB activation of its own upstream activators occurs with every food/butanone training cycle."

load 10 more results