fog-2 : sacy-1

Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results).

fog-3 : sacy-1

Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results). In all female backgrounds tested, we observed oocytes in sacy-1(tm5503) females to undergo meiotic maturation and ovulation at apparently high rates; the uterus filled with unfertilized oocytes (Figure 8A and Figure S3C). We also observed apparently defective ovulation in sacy-1(tm5503) females such that the gonad arms often contained endomitotic oocytes. We confirmed that MSP was undetectable in sacy-1(tm5503) females as expected (Figure S6).

fog-1 : sacy-1

Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results). In all female backgrounds tested,we observed oocytes in sacy-1(tm5503) females to undergo meiotic maturation and ovulation at apparently high rates;the uterus filled with unfertilized oocytes (Figure 8A and Figure S3C). We also observed apparently defective ovulation in sacy-1(tm5503) females such that the gonad arms often contained endomitotic oocytes. We confirmed that MSP was undetectable in sacy-1(tm5503) females as expected (Figure S6).

fem-3 : sacy-1

Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results). In all female backgrounds tested,we observed oocytes in sacy-1(tm5503) females to undergo meiotic maturation and ovulation at apparently high rates;the uterus filled with unfertilized oocytes (Figure 8A and Figure S3C). We also observed apparently defective ovulation in sacy-1(tm5503) females such that the gonad arms often contained endomitotic oocytes. We confirmed that MSP was undetectable in sacy-1(tm5503) females as expected (Figure S6).

bar-1 : pry-1

"Epistasis analysis was performed with pry-1(mu38) and the mutations bar-1(ga80) and mig-14(ga62), which cause too few VPCs to adopt induced fates. The ga80 mutation introduces an early stop codon in BAR-1, and is predicted to cause a null mutant phenotype (Eisenmann et al. 1998). mig-14(ga62) is a viable, reduction-of-function mutation, which causes defects in multiple Wnt-mediated processes in embryogenesis and post-embryonic life (Eisenmann and Kim 2000). The Overinduced phenotype of pry-1(mu38) is still manifest in a double mutant with mig-14(ga62), but is strongly reduced in a double mutant with bar-1(ga80) (Table 1)." With regard to vulval cell induction, pry-1(mu38) is epistatic to mig-14(ga62) and bar-1(ga80) is epistatic to pry-1(mu38).

R06B9.7 : mig-14 : pry-1

"Epistasis analysis was performed with pry-1(mu38) and the mutations bar-1(ga80) and mig-14(ga62), which cause too few VPCs to adopt induced fates. The ga80 mutation introduces an early stop codon in BAR-1, and is predicted to cause a null mutant phenotype (Eisenmann et al. 1998). mig-14(ga62) is a viable, reduction-of-function mutation, which causes defects in multiple Wnt-mediated processes in embryogenesis and post-embryonic life (Eisenmann and Kim 2000). The Overinduced phenotype of pry-1(mu38) is still manifest in a double mutant with mig-14(ga62), but is strongly reduced in a double mutant with bar-1(ga80) (Table 1)." With regard to vulval cell induction, pry-1(mu38) is epistatic to mig-14(ga62) and bar-1(ga80) is epistatic to pry-1(mu38).