"We used miRDeep2 (Friedlander et al. 2012) using miRNA sequences downloaded from miRBase (Kozomara and Griffiths-Jones 2011) to quantify the levels of miRNAs in infected versus uninfected animals... Down-regulated miRNAs included the abundant miRNAs miR-71 and miR-80, which we confirmed via qPCR (Supplemental Fig. 6)."
In wild type, lin-32p::mRFP::unc-60A 3'UTR was intensely detected in every R(n).a + p. However, in plx-1 mutants, R(1).a + p, R(2).a + p and R(5).a + units displayed pronounced decreases in expression of mRFP.
In wild type, lin-32p::mRFP::unc-60A 3'UTR was intensely detected in every R(n).a + p. However, in smp-1smp-2 mutants, R(1).a + p, R(2).a + p and R(5).a + units displayed pronounced decreases in expression of mRFP.
Expression of mec-2-lacZ in the ALML/R, AVM, and PVM touch cells was greatly decreased (<5% of the cells stained); 67% of the PLML/R cells still stained in mec-3(u6).