"The amount of Ct-RBD-1 was very much reduced in the nucleolus, 1 h after the addition of actinomycin D (compare Figure 6, A and B)... After 3 h of actinomycin D treatment, the changes were more accentuated and the nucleolus was almost devoid of Ct-RBD-1 (Figure 6C). In phase contrast, the nucleolus was still detectable after both 1 and 3 h. Ct-RBD-1 is therefore only present in the nucleolus when pre-rRNA is synthesized."
"Following infection, in 45% of worms (n = 120) HIS-24::GFP was found to be present in both the nucleus and cytoplasm as well as in the cytosol (20% of 120 infected worms) (Fig. 7B and C). The observed changes in cellular localization depend on HIS-24 methylation since the subcellular localization of HIS- 24K14A::GFP was unaltered upon infection (Fig. 7D and E)... We found that B. thuringiensis infection enhances considerably the formation of monomethylated histone HIS-24 (2-fold after 12 h and 4-fold after 24 h), whereas infection with the nonpathogenic strain of B. thuringiensis (DSM-350) had almost no effect (Fig. 7G and H). Therefore, bacterial infection strongly influences linker histone methylation."