bbs-5 : daf-19

Expression of the bbs-5::gfp reporter was markedly reduced but not abolished in the absence of DAF-19. Northern blot analysis detected a 1.2 kb transcript. The amount of the 1.2 kb transcript was reduced in daf-19 mutant worms, which confirms the in vivo expression analysis.

ELT-2 binding site : ges-1

The tandem pair of WGATAR sites controlling the Ce- ges-1 gene is indicated ~1.1 kb upstream of the Ce-ges-1 ATG codon.

ELT-2 binding site : ges-1

The tandem pair of WGATAR sites controlling the Ce- ges-1 gene is indicated ~1.1 kb upstream of the Ce-ges-1 ATG codon.

lin-39 : pJW5

A 1.3-kb lin-39 promoter fragment directs GFP expression in the sex myoblasts and in P6.p at the time of vulval induction.

Pair of conserved WGATAR sites : ges-1

The tandem pair of WGATAR sites controlling the Ce- ges-1 gene is indicated ~1.1 kb upstream of the Ce-ges-1 ATG codon.

cdc-25.1 : elt-2

"The injection of a 7.4 kb fragment corresponding to the cdc25.1 gene resulted in the formation of extra intestinal cells in the transformed F1 progeny, while other candidates had no effect (Table 3)."

lin-39 : pJW8

The 1.6-kb first intron of lin-39 directs GFP expression in a subset of VCNs (pJW8; Supplemental Fig. 1; Fig. 1K), which began in the early L2 stage and continued throughout larval life. A minimal 247-bp sequence from lin-39 intron 1 drives expression in a subset of VCNs.

lin-39 : pJW8

The 1.6-kb first intron of lin-39 directs GFP expression in a subset of VCNs (pJW8; Supplemental Fig. 1; Fig. 1K), which began in the early L2 stage and continued throughout larval life. A minimal 247-bp sequence from lin-39 intron 1 drives expression in a subset of VCNs.

daf-16 : vit-2

Deletions of 0.5, 1.2 and 1.7 kb upstream of the vit-2 translational start sequence did not disrupt daf- 16-dependent repression of pvit-2:GFP expression in daf-2(e1370) hermaphrodites. Thus, daf-16 regulation of YP requires only a short 300-bp vit-2 promoter, but not additional upstream sequences.

F45D3.4 : oxygen

F45D3.4 is normally induced in wild-type larvae (L3) when they are subject to 0.1% O2 treatment for 12 hours. Following a 12-hr hypoxic treatment in a chamber containing 0.1% O2, the proximal 3-kb region of the F45D3.4 promoter was sufficient to drive reporter expression in wild-type.