"we analyzed the expression of hsp-12.6, a daf-16 downstream gene, by using qRT-PCR. The results showed that heat stress increased hsp- 12.6 expression (Fig. 2B) as previously reported (Hsu et al., 2003; McColl et al., 2010)."
Feminization with strong loss-of-function mutations in the sex determination pathway delayed the time of onset and the severity of oocyte degeneration in sacy-1(tm5503) females (Figure 8 and Figure S3; S. Kim and D. Greenstein, unpublished results).