"we analyzed the expression of hsp-12.6, a daf-16 downstream gene, by using qRT-PCR. The results showed that heat stress increased hsp- 12.6 expression (Fig. 2B) as previously reported (Hsu et al., 2003; McColl et al., 2010)."
100% of embryos lack endoderm in double mutants at 15 deg C and 25 deg C, whereas lit-1 mutants only exhibit this phenotype at 25 deg C and mom-2 mutants exhibit this phenotype only partially at 22 deg C.
"In wild-type embryos, CeGrip-1 colocalizes with c-tubulin at centrosomes during interphase (unpublished data) and mitosis (Fig. 4 C, left). In gip-1(RNAi) embryos, c-tubulin fails to target to centrosomes (n = 10 embryos; Fig. 4 C, right)"