heat stress : hsp-12.6

"we analyzed the expression of hsp-12.6, a daf-16 downstream gene, by using qRT-PCR. The results showed that heat stress increased hsp- 12.6 expression (Fig. 2B) as previously reported (Hsu et al., 2003; McColl et al., 2010)."

daf-16 : vit-2

Deletions of 0.5, 1.2 and 1.7 kb upstream of the vit-2 translational start sequence did not disrupt daf- 16-dependent repression of pvit-2:GFP expression in daf-2(e1370) hermaphrodites. Thus, daf-16 regulation of YP requires only a short 300-bp vit-2 promoter, but not additional upstream sequences.