efa-6 : zyg-8

To understand how EFA-6 inhibits axon regeneration, authors searched for EFA-6 interacting proteins using yeast two-hybrid screening. We identified two strong interactors, the MT-associated proteins (MAPs) TAC-1 and ZYG-8.

efa-6 : tac-1

To understand how EFA-6 inhibits axon regeneration, authors searched for EFA-6 interacting proteins using yeast two-hybrid screening. We identified two strong interactors, the MT-associated proteins (MAPs) TAC-1 and ZYG-8.

pgrn-1 : ptl-1

We next sought to look at how PGRN interacts with MAPT whose ortholog in C. elegans is ptl-1. We found that the double pgrn-1(tm985); ptl-1(ok621) mutants displayed higher levels of paralysis than ptl-1(ok621) mutants alone, and that the overexpression of PGRN-1 resulted in lower paralysis levels (Fig. 1D).

pgrn-1 : ptl-1

We next sought to look at how PGRN interacts with MAPT whose ortholog in C. elegans is ptl-1. We found that the double pgrn-1(tm985); ptl-1(ok621) mutants displayed higher levels of paralysis than ptl-1(ok621) mutants alone, and that the overexpression of PGRN-1 resulted in lower paralysis levels (Fig. 1D).

Ethylenethiourea : hsp-16.1

"Table 2 also shows how PC72 worms respond to two potentially toxic metabolites of mancozeb, namely inorganic Mn2+ and ethylenethiourea (ETU). Both of these were tested at concentrations up to 10% of the optimal mancozeb dose. At the highest concentration tested (50 g/mL), Mn2+ gives 1.5-fold induction above controls (just significant ;p=0.043), while ETU gives a corresponding figure of 1.8-fold induction (significant; p=0.011)"

Manganese : hsp-16.1

"Table 2 also shows how PC72 worms respond to two potentially toxic metabolites of mancozeb, namely inorganic Mn2+ and ethylenethiourea (ETU). Both of these were tested at concentrations up to 10% of the optimal mancozeb dose. At the highest concentration tested (50 g/mL), Mn2+ gives 1.5-fold induction above controls (just significant ;p=0.043), while ETU gives a corresponding figure of 1.8-fold induction (significant; p=0.011)"

dmd-3 : mab-3 : rme-8

"We also wanted to determine how genes involved in executing morphogenesis might be affected by DMD-3 and MAB-3. In the mab- 3(e1240);dmd-3(ok1327) mutant background, localization of RME-8 is disrupted. RME-8::GFP is still localized to puncta, but it is distributed more evenly in the cytoplasm (in 39/51 males) instead of concentrated near the apical surfaces of the cells (Figure 7A). Thus, dmd-3 and/or mab-3 are upstream of RME-8 localization during tail tip morphogenesis."

cysl-2 : egl-9

To determine how EGL-9 is modulated to control the O2-ON behavior, authors screened for mutants that resembled egl-9 mutants. To facilitate this screen, authors constructed an integrated transgenic reporter strain (nIs470) in which a green fluorescent protein (GFP) variant (Venus) was driven by the promoter of a known HIF-1 target gene, K10H10.2. egl-9 mutants exhibited bright GFP fluorescence throughout the animal, whereas GFP was essentially absent in egl-9(+) and egl-9; hif-1 double mutants, indicating that the GFP transgene specifi- cally reports the transcriptional activity of HIF-1.

daf-16 : single-walled carbon nanotube

"we used daf-16:gfp worms, which contain a transgenic GFP protein fused to DAF-16, to evaluate how a-SWCNT exposure affects the nuclear translocation of DAF-16 in C. elegans. Worms previously treated with or without a-SWCNTs (500 mg/mL) were starved for 2 h to induce DAF-16 nuclear translocation [44]. Under starvation conditions, DAF-16:GFP became localized to the nucleus (Fig. 6D). As expected, a-SWCNTexposed worms displayed a 1.7-fold decrease in nuclear DAF- 16:GFP fluorescence relative to the untreated controls (Fig. 6E), indicating that a-SWCNT treatment inhibited the nuclear translocation of DAF-16 in worms."

arl-1 : dmd-3 : mab-3

"We also wanted to determine how genes involved in executing morphogenesis might be affected by DMD-3 and MAB-3... At early L4, ARL- 1::GFP is expressed intensely in the hyp10 cell in most mab- 3(e1240);dmd-3(ok1327) mutant males (21/34 males), whereas this reporter was expressed intensely in only few wild-type males (4/32; Figure 7B). On the other hand, mutant adults lack the PHCL/R expression (absent in 40/41 males) that is present in all wild-type males (N=35) (Figure 7B). Thus, arl-1 expression is controlled by dmd-3 and/or mab-3, positively in the neurons but negatively in hyp10."