"During these experiments, we also noticed that the DVE-1::GFP fusion protein formed punctate structures in the nucleus during mitochondrial stress (Figures 4B and S4D) (Haynes et al., 2007). In comparison, the intensity of nuclear DVE-1::GFP signal decreased in both lin-65 and met-2 animals fed cco-1 RNAi (Figure S4D) and fewer cells contained detectable DVE-1::GFP puncta (Figures 4E and S4D)."
"During these experiments, we also noticed that the DVE-1::GFP fusion protein formed punctate structures in the nucleus during mitochondrial stress (Figures 4B and S4D) (Haynes et al., 2007). In comparison, the intensity of nuclear DVE-1::GFP signal decreased in both lin-65 and met-2 animals fed cco-1 RNAi (Figure S4D) and fewer cells contained detectable DVE-1::GFP puncta (Figures 4E and S4D)."
100% of embryos lack endoderm in double mutants at 15 deg C and 25 deg C, whereas lit-1 mutants only exhibit this phenotype at 25 deg C and mom-2 mutants exhibit this phenotype only partially at 22 deg C.
"In wild-type embryos, CeGrip-1 colocalizes with c-tubulin at centrosomes during interphase (unpublished data) and mitosis (Fig. 4 C, left). In gip-1(RNAi) embryos, c-tubulin fails to target to centrosomes (n = 10 embryos; Fig. 4 C, right)"