"As a control, it was shown that the Overinduced phenotype caused by an activating mutation in the ras gene, let-60(n1046) (Beitel et al. 1990; Han et al. 1990) is also suppressed by lin- 39(n709ts)."
"... 67% of animals carrying lin-45(n2018cs), a reduction- of-function mutation in the C. elegans raf gene (Han et al. 1993), were Underinduced or Vulvaless, whereas 51% of pry-1(mu38); lin-45(n2018cs) were Overinduced (Table 4; Fig 1)."
"... let-60(n1531) encodes a dominant-negative mutation in let-60 ras (Beitel et al. 1990; Han et al. 1990). A total of 56% of let-60(n1531)/+ animals show a Vulvaless phenotype at 25 degrees Celsius, whereas 38% of pry-1(mu38); let-60(n1531dn) animals show an Overinduced phenotype."
These animals showed no hlh-8::gfp expression throughout the M lineage and lacked all M-derived CCs. This is an enhancement of the defects in the M-lineage compared to either RNAi or mutant alone.
These animals showed no hlh-8::gfp expression throughout the M lineage and lacked all M-derived CCs. This is an enhancement of the defects in the M-lineage compared to either RNAi or mutant alone.