"For lacZ, there is a similarly significant linear trend (p < 0.0001) between b-galactosidase activity and cadmium concentration (Fig. 3B) except that the difference between Cd-exposed and control worms is statistically significant (p < 0.01) even at 5 mg/ml CdCl2... The lacZ reporter product is likewise confined to the pharynx in Cd-exposed worms (shown for 10 mg/ml in Fig. 2h; compare with Fig. 2b), although weaker expression of both reporters can also be seen in other sites (e.g., embryos; not shown) at higher Cd concentrations"
"Green fluorescent protein is strongly induced in heatshocked PC161 worms after 16 h at 30C (Fig. 2c and e) compared with control worms after 16 h at 15C (Fig. 2a)... Nuclear localization of GFP is most obvious in the 20 round intestinal nuclei (green spots in Fig. 2c), probably because these are polyploid and particularly large [33]. The GFP fluorescence is also prominent in the pharynx of heat-shocked worms (not shown) and in developing embryos from gastrulation onward (large green ovoid structures in Fig. 2e);"
"Green fluorescent protein is strongly induced in heatshocked PC161 worms after 16 h at 30C (Fig. 2c and e) compared with control worms after 16 h at 15C (Fig. 2a)... Nuclear localization of GFP is most obvious in the 20 round intestinal nuclei (green spots in Fig. 2c), probably because these are polyploid and particularly large [33]. The GFP fluorescence is also prominent in the pharynx of heat-shocked worms (not shown) and in developing embryos from gastrulation onward (large green ovoid structures in Fig. 2e);"
"Green fluorescent protein is strongly induced in heatshocked PC161 worms after 16 h at 30C (Fig. 2c and e) compared with control worms after 16 h at 15C (Fig. 2a)... Nuclear localization of GFP is most obvious in the 20 round intestinal nuclei (green spots in Fig. 2c), probably because these are polyploid and particularly large [33]. The GFP fluorescence is also prominent in the pharynx of heat-shocked worms (not shown) and in developing embryos from gastrulation onward (large green ovoid structures in Fig. 2e);"
There was a decreased intensity of green fluorescent protein (GFP) signal in worms fed with OxyR or OxyS strain compared with K12. Western blots confirmed this reduction.
"There is a highly significant linear trend (p < 0.0001) between GFP fluorescence and CdCl2 concentration (Fig. 3A)... fluorescence microscopy shows that GFP is visibly induced by Cd at concentrations of 100, 50, and even 10 mg/ml, but its expression is limited essentially to the pharynx (shown for 10 mg/ml in Fig. 2g; compare with Fig. 2a)."