In reproductively growing animals, in which IIS is high, DAF-16::GFP is cytoplasmic, whereas in animals with reduced IIS, it is found predominantly in the nucleus. In starved L1 larvae, DAF-16::GFP is nuclearIn 25% of
asna-1(
sv42) adults, DAF-16::GFP was nuclear (cf. 0% for well-fed
asna-1(+) animals, n > 100). Nuclear DAF-16::GFP was also seen in 82% of L1 arrested
asna-1(RNAi) animals.