"We examined GFP expression in hsp-4::GFP worms by Western blotting (Figure 8B). erl-1(tm2703) had no apparent effect on GFP expression either under basal conditions or following exposure to 5 μg/ml TN for various lengths of time."
36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. Among them, we found many components of the core translational initiation complex, including PAB-1 and PAB-2 (the two C. elegans cytoplasmic polyadenylate-binding proteins, or PABPC [Mangus et al., 2003]), IFG-1 (the C. elegans ortholog of eIF4G [Dinkova et al., 2005]), and RPS-0 (a component of the 40S ribosome subunit [Ford et al., 1999]).
36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. Among them, we found many components of the core translational initiation complex, including PAB-1 and PAB-2 (the two C. elegans cytoplasmic polyadenylate-binding proteins, or PABPC [Mangus et al., 2003]), IFG-1 (the C. elegans ortholog of eIF4G [Dinkova et al., 2005]), and RPS-0 (a component of the 40S ribosome subunit [Ford et al., 1999]).
36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. Among them, we found many components of the core translational initiation complex, including PAB-1 and PAB-2 (the two C. elegans cytoplasmic polyadenylate-binding proteins, or PABPC [Mangus et al., 2003]), IFG-1 (the C. elegans ortholog of eIF4G [Dinkova et al., 2005]), and RPS-0 (a component of the 40S ribosome subunit [Ford et al., 1999]).
36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. Among them, we found many components of the core translational initiation complex, including PAB-1 and PAB-2 (the two C. elegans cytoplasmic polyadenylate-binding proteins, or PABPC [Mangus et al., 2003]), IFG-1 (the C. elegans ortholog of eIF4G [Dinkova et al., 2005]), and RPS-0 (a component of the 40S ribosome subunit [Ford et al., 1999]).