"We have shown that the
hlh-8 (
n2170) strain is a semidominant allele... We observed the uterine muscles with
rgs-2::gfp (regulator of G protein signaling) (Dong et al., 2000), and we found wild-type,
nr2061 (-)/+ and
n2170sd/+ heterozygotes to be indistinguishable (Fig. 7D,H,L). In wildtype animals, the four
vm1 type vulval muscles express
egl15::gfp (Harfe et al., 1998a). Using the
egl-15::gfp reporter, we observed that
n2170sd/+ heterozygotes never expressed GFP from the
egl-15 promoter in the
vm1 vulval muscles (n>50), whereas wild-type and
nr2061 (-)/+ heterozygotes always expressed the reporter in the
vm1 cells (n>50) (Fig. 7B,F,J)."