In a strong gld-2 loss-of-function allele(q497) CGH-1 was appropriately upregulated at meiosis entr y and appeared to be localized normally through the pachytene stage, but expression of both CGH-1 and PGL-1 was lost more proximally, in the abnormal gametes that are produced.
In a strong gld-2 loss-of-function allele(q497) CGH-1 was appropriately upregulated at meiosis entr y and appeared to be localized normally through the pachytene stage, but expression of both CGH-1 and PGL-1 was lost more proximally, in the abnormal gametes that are produced.
In a strong gld-2 loss-of-function allele(q497) CGH-1 was appropriately upregulated at meiosis entr y and appeared to be localized normally through the pachytene stage, but expression of both CGH-1 and PGL-1 was lost more proximally, in the abnormal gametes that are produced.
In a strong gld-2 loss-of-function allele(q497) CGH-1 was appropriately upregulated at meiosis entr y and appeared to be localized normally through the pachytene stage, but expression of both CGH-1 and PGL-1 was lost more proximally, in the abnormal gametes that are produced.
In a strong gld-2 loss-of-function allele(q497) CGH-1 was appropriately upregulated at meiosis entr y and appeared to be localized normally through the pachytene stage, but expression of both CGH-1 and PGL-1 was lost more proximally, in the abnormal gametes that are produced.
The mec-6 gene is required for the punctate distribution of MEC-4. When mec-4::yfp and promoter mec-4::cfp were injected into mec-6(u3) animals, the expression of the promoter mec-4::cfp fusion was unchanged, but the punctate expression from mec-4::y fp was undetectable.
Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs.
Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs.