The dafachronic acids (DAs) and their proposed precursors were fed to daf-36 mutants. Mutants cultured on vehicle resulted in upregulation of hypodermal daf-9::gfp compared to WT controls.
"When e2322ts animals are maintained at the permissive temperature (16C) throughout development, both the DA and VA motor neurons express high levels of UNC-17 protein (Table 3). Growth at the restrictive temperature (25C) results in substantially lower levels of UNC-17 expression (18% of DAs and 6% of VAs)."