"To examine the expression pattern and localization of CSR-1 during male gametogenesis, we performed immunofluorescence using an antibody that recognizes endogenous CSR-1 (Claycomb et al., 2009). CSR-1 was associated with P-granules throughout the syncytial male germline and into differentiating spermatocytes, where P-granules disperse and disappear. In developing gametes, we found that CSR-1 localized to large cytoplasmic foci and in discrete chromatin domains of spermatocytes undergoing nuclear condensation as well as in haploid spermatids (Figure 5B). In
alg-3/4 mutants cultured at 25C, CSR-1 was present in large cytoplasmic foci in spermatocytes undergoing nuclear condensation but was largely absent from chromatin. Notably, in haploid spermatids from
alg-3/4 males, we observed fewer CSR-1 cytoplasmic and nuclear foci than in haploid spermatids from WT males (Figure 5B)."