4-cell embryos exposed to anoxia contain detectable SAN-1 localized to the kinetochore and in lateral projections from the metaphase plate. There is less detectable SAN-1 in the nucleoplasm in metaphase blastomeres of embryos exposed to 30 minutes or 6 hours of anoxia in comparison to normoxic embryos. The lateral projections were not as easily detected in 8-cell embryos exposed to 6 hours or more of anoxia suggesting that either the kinetochore projections are only a characteristic of 4-cell embryos or the projections are more difficult to detect in embryos developed beyond the 4-cell stage. Embryos exposed to 12 hours or more of anoxia contains SAN-1 localized in punctate form in the nucleoplasm surrounding the metaphase plate. Embryos exposed to at least 24 hours of anoxia display a reduction in the detection of SAN-1 at the kinetochore. Taken together, it is concluded that SAN-1 localization changes are dependent upon anoxia exposure time.
4-cell embryos exposed to anoxia contain detectable SAN-1 localized to the kinetochore and in lateral projections from the metaphase plate. There is less detectable SAN-1 in the nucleoplasm in metaphase blastomeres of embryos exposed to 30 minutes or 6 hours of anoxia in comparison to normoxic embryos. The lateral projections were not as easily detected in 8-cell embryos exposed to 6 hours or more of anoxia suggesting that either the kinetochore projections are only a characteristic of 4-cell embryos or the projections are more difficult to detect in embryos developed beyond the 4-cell stage. Embryos exposed to 12 hours or more of anoxia contains SAN-1 localized in punctate form in the nucleoplasm surrounding the metaphase plate. Embryos exposed to at least 24 hours of anoxia display a reduction in the detection of SAN-1 at the kinetochore. Taken together, it is concluded that SAN-1 localization changes are dependent upon anoxia exposure time.