RNAi depletion of CAR-1 caused ectopic GLP-1 protein expression in oocytes of wild-type gonads. Gonads homozygous for the
car-1(
tm1753) deletion also exhibited ectopic GLP-1 expression only in oocytes with 100% penetrance.
glp-1 mRNA levels were similar in
car-1(
tm1753) mutant and wild-type animals, which supports CAR-1 control of
glp-1 translation rather than mRNA production or stability.