"In two models of mitochondrial stress, feeding of
spg-7(RNAi) as well as a previously characterized temperature-sensitive allele (
zc32) (Benedetti et al., 2006), GFP fluorescence increased over the course of postembryonic development. The increase in fluorescence and GFP levels was attenuated in
haf-1(
ok705) deletion animals (Figures 1A and 1B). Inhibition of the UPRmt reporter was mirrored by the effects of
haf-1(
ok705) and
haf-1(
tm843) deletions on the expression of the endogenous mitochondrial chaperone gene
hsp-60, as determined by quantitative RT-PCR (Figure 1C). Similar results were obtained for the mitochondrial HSP70 gene,
hsp-6 (Figures S1C and S1D)."