[cgc6390]:Cluster_F

hierarchical clustering Germline-enriched and sex-biased expression profile cluster F.

WBPaper00038168:lin-35_downregulated

For each gene in each microarray hybridization experiment, the ratio of RNA levels from the two samples was transformed into a log2 value and the mean log2 ratio was calculated. The log2 ratios were normalized by print-tip Loess normalization (Dudoit and Yang, 2002). All genes with a false discovery rate of <= 5% (q <= 0.05) (Storey and Tibshirani, 2003) and a mean fold-change ratio of >= 1.5 were selected for further analysis. Genes that were downregulated in lin-35.

WBPaper00038168:lin-35_upregulated

For each gene in each microarray hybridization experiment, the ratio of RNA levels from the two samples was transformed into a log2 value and the mean log2 ratio was calculated. The log2 ratios were normalized by print-tip Loess normalization (Dudoit and Yang, 2002). All genes with a false discovery rate of <= 5% (q <= 0.05) (Storey and Tibshirani, 2003) and a mean fold-change ratio of >= 1.5 were selected for further analysis. Genes that were upregulated in lin-35.

WBPaper00038168:lin-15B(n744)_downregulated

For each gene in each microarray hybridization experiment, the ratio of RNA levels from the two samples was transformed into a log2 value and the mean log2 ratio was calculated. The log2 ratios were normalized by print-tip Loess normalization (Dudoit and Yang, 2002). All genes with a false discovery rate of <= 5% (q <= 0.05) (Storey and Tibshirani, 2003) and a mean fold-change ratio of >= 1.5 were selected for further analysis. Genes that were downregulated in lin-15B(n744).

WBPaper00038168:lin-15B(n744)_upregulated

For each gene in each microarray hybridization experiment, the ratio of RNA levels from the two samples was transformed into a log2 value and the mean log2 ratio was calculated. The log2 ratios were normalized by print-tip Loess normalization (Dudoit and Yang, 2002). All genes with a false discovery rate of <= 5% (q <= 0.05) (Storey and Tibshirani, 2003) and a mean fold-change ratio of >= 1.5 were selected for further analysis. Genes that were upregulated in lin-15B(n744).

WBPaper00024704:Soluble_protein

N.A. Soluble proteins identified according to HPLC electrospray ionization (ESI)-MS-MS, using the method in which the protein mixtures were first derivatized with a water-soluble fluorogenic reagent, SBD-F or 7-chloro-4-(dimethylaminoethylaminosulfonyl)-2,1,3-benzoxadiazole (DAABD-Cl).

WBPaper00061340:F_U

Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm. Top 300 transcripts enriched in F cell, U cell according to single cell RNAseq.

WBPaper00061340:B_F_K_Kp_U_Y

Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm. Top 300 transcripts enriched in B cell, F cell, K cell, K' cell, U cell, Y cell according to single cell RNAseq.

[cgc5767]:expression_class_ET_max(53_min)

A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing. Embryonic transient (ET) subclasses are based on time of max abundance.

[cgc5767]:expression_class_ET_max(23_min)

A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing. Embryonic transient (ET) subclasses are based on time of max abundance.