WBPaper00052959:hsf-1(RNAi)_regulated_miRNA_HS-dependent

The Benjamini-Hochberg correction for multiple testing was then used to determine significantly altered miRNAs between each treatment condition. Micro RNAs with q-value < 0.05 was considered differentially expressed. MicroRNAs that showed significantly altered expression in hsf-1(+);+HS vs hsf-1(RNAi);+HS. Worms remained on RNAi plates until the L4 larval-stage before being heat shocked by submerging plates in a 33C water bath for 30 minutes.

WBPaper00061341:28C_48h_upregulated

Differentially expressed genes wereidentified using DESeq (v.1.18.0) by normalizing readsbased on the negative binomial distribution method andcomparing each HS timepoint to the 0-h control. Transcripts that showed significantly increased expression after animals were exposed to 28C temperature for 48 hours.

WBPaper00061341:28C_48h_downregulated

Differentially expressed genes wereidentified using DESeq (v.1.18.0) by normalizing readsbased on the negative binomial distribution method andcomparing each HS timepoint to the 0-h control. Transcripts that showed significantly decreased expression after animals were exposed to 28C temperature for 48 hours.

WBPaper00061341:28C_24h_downregulated

Differentially expressed genes wereidentified using DESeq (v.1.18.0) by normalizing readsbased on the negative binomial distribution method andcomparing each HS timepoint to the 0-h control. Transcripts that showed significantly decreased expression after animals were exposed to 28C temperature for 24 hours.

WBPaper00061341:28C_1h_upregulated

Differentially expressed genes wereidentified using DESeq (v.1.18.0) by normalizing readsbased on the negative binomial distribution method andcomparing each HS timepoint to the 0-h control. Transcripts that showed significantly increased expression after animals were exposed to 28C temperature for 1 hour.

WBPaper00061341:28C_1h_downregulated

Differentially expressed genes wereidentified using DESeq (v.1.18.0) by normalizing readsbased on the negative binomial distribution method andcomparing each HS timepoint to the 0-h control. Transcripts that showed significantly decreased expression after animals were exposed to 28C temperature for 1 hour.

WBPaper00061341:28C_24h_upregulated

Differentially expressed genes wereidentified using DESeq (v.1.18.0) by normalizing readsbased on the negative binomial distribution method andcomparing each HS timepoint to the 0-h control. Transcripts that showed significantly increased expression after animals were exposed to 28C temperature for 24 hours.

WBPaper00049942:hsf-1(RNAi)_upregulated

Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value). Transcripts that showed significantly increased expression in hsf-1(RNAi) animals comparing to in control animals, without heat shock.

WBPaper00049942:hsf-1(RNAi)_downregulated

Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value). Transcripts that showed significantly decreased expression in hsf-1(RNAi) animals comparing to in control animals, without heat shock.

WBPaper00049942:HeatShock_upregulated_hsf-1(+)

Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value). Transcripts that showed significantly increased expression in animals injected with control vector, after heat shock at 33 C for 30min, comparing to without heat shock.