WBPaper00053420:Microgravity-Simulated_downregulated Transcriptome Analysis Console version 3.0 software (Affymetrix, CA, USA) for differential gene expression analysis. Selection of differentially expressed genes was basd on filter criteria which were defined as one-way ANOVA p-value of < 0.05 with fold changes of <2 and >2.
Gene that showed significantly decreased expression by microgravity simulation using a desktop RPM, from hatching to L4 larva stage.
WBPaper00053420:Microgravity-Simulated_upregulated Transcriptome Analysis Console version 3.0 software (Affymetrix, CA, USA) for differential gene expression analysis. Selection of differentially expressed genes was basd on filter criteria which were defined as one-way ANOVA p-value of < 0.05 with fold changes of <2 and >2.
Genes that showed significantly increased expression by microgravity simulation using a desktop RPM, from hatching to L4 larva stage.
WBPaper00040185:nhr-23(RNAi)_downregulated Microarray chip data was collected and analyzed by both Affymetrix MAS 5.0 suite software (>= 1.6-fold change in mRNA expression) and Robust Multichip Average (RMA) (>= 1.2-fold change in mRNA expression) as part of the Partek genomics suite software package, all with a p-value less than or equal to 0.05. Normalized data was further analyzed and visualized with Genespring software (Agilent Technologies, Santa Clara, CA).
Genes that showed decreased expression in nhr-23 RNAi experiment.
WBPaper00038304:octr-1_regulated Microarray data were subjected to the robust multichip averaging algorithm using GeneSpring GX software (Agilent Technologies, Santa Clara, CA). Analysis of variance t test and fold-change calculations were also performed using GeneSpring GX software. Probability calculations of enrichment were performed using the hypergeometric probability test (http://elegans.uky.edu/MA/progs/overlap_stats.html) to calculate the statistical significance of the overlap of gene groups.
Genes regulated by octr-1(ok371) after infected with P. aeruginosa PA14 for 4 hours at 25 centigrade.