WBPaper00060871:M.humicola-infection_12h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 12 hours after animals were infected by M. humicola.

WBPaper00060871:M.humicola-infection_24h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 24 hours after animals were infected by M. humicola.

WBPaper00060871:M.humicola-infection_48h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 48 hours after animals were infected by M. humicola.

WBPaper00060871:M.humicola-extract_24h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 24 hours after animals were exposed to M. humicola extract.

WBPaper00060871:M.humicola-extract_12h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 12 hours after animals were exposed to M. humicola extract.

WBPaper00060871:M.humicola-extract_1h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression one hour after animals were exposed to M. humicola extract.

WBPaper00060871:M.humicola-extract_4h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 4 hours after animals were exposed to M. humicola extract.

WBPaper00061340:M_cell

Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm. Top 300 transcripts enriched in M cell according to single cell RNAseq.

WBPaper00050332:juglone_upregulated

Using the Cufflink package and CuffDiff application from Galaxy, FPKM (Fragments Per Kilobase of transcript per Million mapped reads) were calculated and tested for differential expression with a FDR score of 5%. Transcripts that showed significantly increased expression after exposed to 38 M juglone for 3 hours.

WBPaper00037950:intestine_L2-larva_expressed

A Mann-Whitney U test with an empirical background model and FDR correction for multiple testing was used to detect expressed transcripts (Benjamini and Hochberg 1995). Genes and TARs with an FDR <= 0.05 were reported as expressed above background. Authors detected differentially expressed transcripts using a method based on linear models. Genes and TARs were called differentially expressed if the FDR was <= 0.05 and the fold change (FC) >= 2.0. To more strictly correct for potential false-positives resulting from multiple sample comparisons, authors divided individual FDR estimates by the number of samplesor sample comparisons, respectively. This resulted in an adjusted FDR of 1.3 * 0.0001 for expression above background and of 7.4 * 0.0001 for differential expression. Authors called genes selectively enriched in a given tissue if they met the following requirements: (1) enriched expression in a given tissue (FDR <= 0.05 and FC >= 2.0), (2) fold change versus reference among the upper 40% of the positive FC range observed for this gene across all tissues, and (3) fold-change entropy among the lower 40% of the distribution observed for all genes. Genes that showed expression levels higher than the corresponding reference sample (L2 all cell reference).