- WBPaper00041207:CE_dauer_down
The weight parameters were optimized based on MA-plots such that spike-in controls show their expected fold change values. lmFit function was used to fit a linear model to probe intensities across arrays, and differential expression was calculated by empirical Bayes method using the eBayes function. Control of FDR was employed as correction for multiple testing.
Genes dowm regulated in the dauer versus dauer-exit worms.
- WBPaper00041207:CE_dauer_up
The weight parameters were optimized based on MA-plots such that spike-in controls show their expected fold change values. lmFit function was used to fit a linear model to probe intensities across arrays, and differential expression was calculated by empirical Bayes method using the eBayes function. Control of FDR was employed as correction for multiple testing.
Genes up regulated in the dauer versus dauer-exit worms.
- WBPaper00038304:octr-1_regulated
Microarray data were subjected to the robust multichip averaging algorithm using GeneSpring GX software (Agilent Technologies, Santa Clara, CA). Analysis of variance t test and fold-change calculations were also performed using GeneSpring GX software. Probability calculations of enrichment were performed using the hypergeometric probability test (http://elegans.uky.edu/MA/progs/overlap_stats.html) to calculate the statistical significance of the overlap of gene groups.
Genes regulated by octr-1(ok371) after infected with P. aeruginosa PA14 for 4 hours at 25 centigrade.
- WBPaper00032022:X-Ray_upregulated
Gene was considered induced if the difference in expression levels was significant (p < 0.05) compared to the control. To be considered the change in expression had to be at least 2 fold compared to the control.
Genes induced at least 2 fold 2 hours after 120Gy X-ray treatment
- WBPaper00034739:TreatmentAffectedGenes
The normalised data were analysed using a two-way ANOVA, testing for each gene the effects of LINE (N2, DR1350, RIL-14, RIL-17), TREATMENT (non-dauer vs dauer larva-inducing) and the LINE TREATMENT interaction, using a published PERL script.
Genes with expression significantly affected by TREATMENT (non-dauer vs dauer), or TREATMENT X LINE at p < 0.001.
- WBPaper00054191:H3.3(null)_embryo_downregulated
Differentially expressed genes were estimated using a General Linear Model approach, negative binomial distribution, and a quasi-likelihood F-test. Genes with a P-value < 0.05 and fold change > 1 were considered significant.
Transcripts that showed significantly decreased expression in H3.3 null mutant animals (FAS43 [his-69&his-70(uge44) III; his-72(tm2066) III; his-74(uge18) V; his-71(ok2289) X]) comparing to in N2 animals at embryo stage.
- WBPaper00054191:H3.3(null)_embryo_upregulated
Differentially expressed genes were estimated using a General Linear Model approach, negative binomial distribution, and a quasi-likelihood F-test. Genes with a P-value < 0.05 and fold change > 1 were considered significant.
Transcripts that showed significantly increased expression in H3.3 null mutant animals (FAS43 [his-69&his-70(uge44) III; his-72(tm2066) III; his-74(uge18) V; his-71(ok2289) X]) comparing to in N2 animals at embryo stage.
- WBPaper00054191:H3.3(null)_L1_downregulated
Differentially expressed genes were estimated using a General Linear Model approach, negative binomial distribution, and a quasi-likelihood F-test. Genes with a P-value < 0.05 and fold change > 1 were considered significant.
Transcripts that showed significantly decreased expression in H3.3 null mutant animals (FAS43 [his-69&his-70(uge44) III; his-72(tm2066) III; his-74(uge18) V; his-71(ok2289) X]) comparing to in N2 animals at L1 larva stage.
- WBPaper00054191:H3.3(null)_L1_upregulated
Differentially expressed genes were estimated using a General Linear Model approach, negative binomial distribution, and a quasi-likelihood F-test. Genes with a P-value < 0.05 and fold change > 1 were considered significant.
Transcripts that showed significantly increased expression in H3.3 null mutant animals (FAS43 [his-69&his-70(uge44) III; his-72(tm2066) III; his-74(uge18) V; his-71(ok2289) X]) comparing to in N2 animals at L1 larva stage.