WBPaper00041506:AB_enriched

t-test with p-value < 0.05 (FDR corrected) Transcripts enriched in emrbyonic AB cell comparing to in P1, according to single cell RNAseq.

WBPaper00041506:P1_enriched

t-test with p-value < 0.05 (FDR corrected) Transcripts enriched in emrbyonic P1 cell comparing to in AB, according to single cell RNAseq.

WBPaper00046691:P1_enriched

Reads were called as present if the number of mapped reads met a minimum 50 reads in the mean of all samples and at least 1 read in the mean of either AB or P1 samples. Authors identified 7945 present genes of 20,240 of the predicted gene models. Present genes were called as giving rise to significantly asymmetric transcripts if they met a P-value < 0.1 after FDR adjustment using the Benjamani-Hochberg method within the DEseq package in R 2.12 (All other R analyses were performed in 3.0). When necessary, genes were ranked by negative log P-value. Transcripts enriched in embryonic P1 cell comparing to in embryonic AB cell, according to RNAseq analysis performed on dissected blastomere.

WBPaper00046691:AB_enriched

Reads were called as present if the number of mapped reads met a minimum 50 reads in the mean of all samples and at least 1 read in the mean of either AB or P1 samples. Authors identified 7945 present genes of 20,240 of the predicted gene models. Present genes were called as giving rise to significantly asymmetric transcripts if they met a P-value < 0.1 after FDR adjustment using the Benjamani-Hochberg method within the DEseq package in R 2.12 (All other R analyses were performed in 3.0). When necessary, genes were ranked by negative log P-value. Transcripts enriched in embryonic AB cell comparing to in embryonic P1 cell, according to RNAseq analysis performed on dissected blastomere.

WBPaper00046121:ecdoderm_unique

Germ layers were assigned by correlating the average expression with germ-layer-specific patterns with a cutoff of 0.6 correlation with the following idealized vectors: endoderm = [00100]; ectoderm = [10000]; mesoderm = [01011], where the order is AB, MS, E, C and P3. Germ-layer genes were defined according to the sum of the genes identified by the clusters and are indicated in Fig. 2b. Authors further filtered the germ-layer gene sets by keeping only those genes whose expression was partitioned across the germ layers such that at least two-thirds of the expression was in that germ layer. Genes uniquely expressed in ecdoderm, according to RNAseq studies on blastomere (with isolated AB, MS, E, C, D founder cells dividing in vitro) time course and whole embryo time course.

WBPaper00046121:endoderm_unique

Germ layers were assigned by correlating the average expression with germ-layer-specific patterns with a cutoff of 0.6 correlation with the following idealized vectors: endoderm = [00100]; ectoderm = [10000]; mesoderm = [01011], where the order is AB, MS, E, C and P3. Germ-layer genes were defined according to the sum of the genes identified by the clusters and are indicated in Fig. 2b. Authors further filtered the germ-layer gene sets by keeping only those genes whose expression was partitioned across the germ layers such that at least two-thirds of the expression was in that germ layer. Genes uniquely expressed in endoderm, according to RNAseq studies on blastomere (with isolated AB, MS, E, C, D founder cells dividing in vitro) time course and whole embryo time course.

WBPaper00046121:mesoderm_unique

Germ layers were assigned by correlating the average expression with germ-layer-specific patterns with a cutoff of 0.6 correlation with the following idealized vectors: endoderm = [00100]; ectoderm = [10000]; mesoderm = [01011], where the order is AB, MS, E, C and P3. Germ-layer genes were defined according to the sum of the genes identified by the clusters and are indicated in Fig. 2b. Authors further filtered the germ-layer gene sets by keeping only those genes whose expression was partitioned across the germ layers such that at least two-thirds of the expression was in that germ layer. Genes uniquely expressed in mesoderm, according to RNAseq studies on blastomere (with isolated AB, MS, E, C, D founder cells dividing in vitro) time course and whole embryo time course.