WBPaper00060014:set-2(tm1630)_upregulated

DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. Transcripts that showed significantly increased expression in set-2(tm1630) animals at embryo stage, comparing to in N2 animals.

WBPaper00060014:set-2(zr2012)_downregulated

DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. Transcripts that showed significantly decreased expression in set-2(zr2012) animals at embryo stage, comparing to in N2 animals.

WBPaper00060014:set-2(zr2012)_upregulated

DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. Transcripts that showed significantly increased expression in set-2(zr2012) animals at embryo stage, comparing to in N2 animals.

WBPaper00060014:set-2(tm1630)_downregulated

DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. Transcripts that showed significantly decreased expression in set-2(tm1630) animals at embryo stage, comparing to in N2 animals.

WBPaper00029047:atx-3_downregulated

Differentially expressed (DE) genes were selected using a Bayesian approach with a false discovery rate of 0.1%. Genes that showed significantly decreased expression in both atx-3(gk193) and atx-3(tm1689) comparing with N2.

WBPaper00029047:atx-3_upregulated

Differentially expressed (DE) genes were selected using a Bayesian approach with a false discovery rate of 0.1%. Genes that showed significantly increased expression in both atx-3(gk193) and atx-3(tm1689) comparing with N2.

WBPaper00044027:tyrosol_regulated

The definitive selection of the spots differentially expressed was made using the one-way ANOVA followed by the Students t-test. Proteins that showed significantly altered expression between animals grown with or without 250uM tyrosol, according to 2-DE.

WBPaper00056474:ivermectin-resistent_vs_N2_upregulated_protein

p < 0.05 Proteins that showed significantly lower expression in ivermectin-resistent strain comparing to in N2, according to 2-DE and MS analysis. Ivermectin resistent strains were develped stepwise in plates contaings NGM with the addition of doses (1ng per ml)of ivermectin.

WBPaper00056474:ivermectin-resistent_vs_N2_downregulated_protein

p < 0.05 Proteins that showed significantly lower expression in ivermectin-resistent strains comparing to in N2, according to 2-DE and MS analysis. Ivermectin resistent strains were develped stepwise in plates contaings NGM with the addition of doses (1ng per ml)of ivermectin.

WBPaper00046415:lithium_downregulated

All data were analyzed using Stat View J 5.0, with all experimental data checked for assumptions of homogeneity of variance across manipulations using Bartletts test. Once the assumptions were satisfied, the data were analyzed by one-way analysis of variance followed by Dunnetts multiple comparison test. When homogeneity was not evident in the data, we used the nonparametric KruskalWallis test, followed by the MannWhitney U-test with a Bonferroni adjustment. Differences were considered significant at P < 0.05. Genes suppressed following 24h exposure to lithium compounds (78uM LiCl and 375uM Li2CO3), according to custom DNA microarray.