WBPaper00060871:M.humicola-infection_12h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 12 hours after animals were infected by M. humicola.

WBPaper00060871:M.humicola-infection_24h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 24 hours after animals were infected by M. humicola.

WBPaper00060871:M.humicola-infection_48h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 48 hours after animals were infected by M. humicola.

WBPaper00060871:M.humicola-extract_24h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 24 hours after animals were exposed to M. humicola extract.

WBPaper00060871:M.humicola-extract_12h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 12 hours after animals were exposed to M. humicola extract.

WBPaper00060871:M.humicola-extract_1h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression one hour after animals were exposed to M. humicola extract.

WBPaper00060871:M.humicola-extract_4h_regulated

Differentially expressed genes as determined by Kallisto and Sleuth (pval<0.01, qval<0.1). Transcripts that showed significantly altered expression 4 hours after animals were exposed to M. humicola extract.

WBPaper00061340:M_cell

Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm. Top 300 transcripts enriched in M cell according to single cell RNAseq.

WBPaper00050332:juglone_upregulated

Using the Cufflink package and CuffDiff application from Galaxy, FPKM (Fragments Per Kilobase of transcript per Million mapped reads) were calculated and tested for differential expression with a FDR score of 5%. Transcripts that showed significantly increased expression after exposed to 38 M juglone for 3 hours.

WBPaper00041002:HF_3d_0.2mM_Up

For selection of DEGs, an unpaired t -test was performed followed by a significance analysis of microarray (SAM) test including a calculation that estimates the false discovery rate (FDR). FDR, reducing on the one hand type I errors for null associations, was set to a non-stringent level of <12.5%, mainly to guard from an increase of type II error and also based on findings by Levine et al. (2011), which described 12.5% as most acceptable optimum level of FDR, representing the 90th percentile of the normal distribution curve. DEGs exceeding a fold change of 1.25 were further analyzed with respect to their functional clustering. This fold-cut-off was chosen to allow an interpretation that is biologically meaningful, akin to the notion that data of sound technical and experimental quality which returns strong, statistically significant, absolute signal intensities is sufficiently robust to justify a fold-cut-off of >1.2. This analysis was conducted using the functional annotation clustering tool of the Database for Annotation, Visualization, and Integrated Discovery (DAVID; Huang et al., 2007). Gene significantly up-regulated by treatment with 0.2mM of HuminFeed until young adult stage (3 days), with a minimum fold change in gene expression of 1.25.