WBPaper00060014:set-2(tm1630)_upregulated

DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. Transcripts that showed significantly increased expression in set-2(tm1630) animals at embryo stage, comparing to in N2 animals.

WBPaper00060014:set-2(zr2012)_downregulated

DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. Transcripts that showed significantly decreased expression in set-2(zr2012) animals at embryo stage, comparing to in N2 animals.

WBPaper00060014:set-2(zr2012)_upregulated

DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. Transcripts that showed significantly increased expression in set-2(zr2012) animals at embryo stage, comparing to in N2 animals.

WBPaper00060014:set-2(tm1630)_downregulated

DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. Transcripts that showed significantly decreased expression in set-2(tm1630) animals at embryo stage, comparing to in N2 animals.

WBPaper00029047:atx-3_downregulated

Differentially expressed (DE) genes were selected using a Bayesian approach with a false discovery rate of 0.1%. Genes that showed significantly decreased expression in both atx-3(gk193) and atx-3(tm1689) comparing with N2.

WBPaper00029047:atx-3_upregulated

Differentially expressed (DE) genes were selected using a Bayesian approach with a false discovery rate of 0.1%. Genes that showed significantly increased expression in both atx-3(gk193) and atx-3(tm1689) comparing with N2.

WBPaper00044027:tyrosol_regulated

The definitive selection of the spots differentially expressed was made using the one-way ANOVA followed by the Students t-test. Proteins that showed significantly altered expression between animals grown with or without 250uM tyrosol, according to 2-DE.

WBPaper00056474:ivermectin-resistent_vs_N2_upregulated_protein

p < 0.05 Proteins that showed significantly lower expression in ivermectin-resistent strain comparing to in N2, according to 2-DE and MS analysis. Ivermectin resistent strains were develped stepwise in plates contaings NGM with the addition of doses (1ng per ml)of ivermectin.

WBPaper00056474:ivermectin-resistent_vs_N2_downregulated_protein

p < 0.05 Proteins that showed significantly lower expression in ivermectin-resistent strains comparing to in N2, according to 2-DE and MS analysis. Ivermectin resistent strains were develped stepwise in plates contaings NGM with the addition of doses (1ng per ml)of ivermectin.

WBPaper00046421:cdc-48.2(tm659)_upregulated

Data are presented as mean SEM of at least three separate experiments if not specified otherwise and compared with multiple t-test corrected using the Holm-Sidak method for C. elegans experiments or the one-way analysis of variance (ANOVA) followed by Dunnett's multiple comparison tests for experiments on mammalian cells. The significance was set at P value < 0.05. All statistical analyses were performed using GraphPad Prism (version 6) statistical software (GraphPad Software; San Diego). Proteins that showed significantly increased expression in cdc-48.2 (EM2[dpy-5(e907); cdc-48.2(tm659); sIs13872[ckb-2p::gfp, pCeh361]]) comparing to in WT (BC14636[dpy-5(e907); sIs13872[ckb-2p::gfp, pCeh361]]) upon tunicamycin treatment.