WBPaper00044452:ace_1_ace-2_upregulated

Proteins that showed more than 4 fold change in expression are considered differentially expressed. Proteins that showed increased expression for more than 4 fold in ace-1(p1000);ace-2(g72) comparing to in N2, according to 2DE LS-MS/MS analysis.

WBPaper00044452:ace_1_ace-2_downregulated

Proteins that showed more than 4 fold change in expression are considered differentially expressed. Proteins that showed decreased expression for more than 4 fold in ace-1(p1000);ace-2(g72) comparing to in N2, according to 2DE LS-MS/MS analysis.

WBPaper00064637:cox-5B(RNAi)_upregulated

LimmaVoom. The genes with a BenjaminiHochbergadjusted P value < 0.05 were defined as statistically significant. Genes whose expressions were significantly upregulated with log2FC > 0.393. Transcripts that showed significantly increased expression in cco-1/cox-5B(RNAi) animals comparings to animals injected with vector control.

WBPaper00066447:Day8_vs_Day1_downregulated_coelomocyte

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in coelomocyte.

WBPaper00066447:Day8_vs_Day1_downregulated_intestine

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in intestine.

WBPaper00046497:B.thuringiensis_downregulated_protein

Quantitative results of log2 transformed iTRAQ-protein ratios describing the condition pathogenic/non-pathogenic BT were tested against all log2 transformed control-ratios of biological replicates in the same iTRAQ experiment using the two sided Welchs t-test. To adjust for multiple testing, p-values were corrected by permutation based FDR approach with 1000 randomizations. Protein groups with log2 ratios of at least +/- 0.485 (which corresponds to iTRAQ-ratios >= 1.4 or <= 0.71) with FDR-corrected p-value <= 0.05 were considered to be differentially expressed. Proteins that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT, according to proteomic studies via isobaric labelling and 2D-LS-MS/MS.

WBPaper00066447:Day8_vs_Day1_upregulated_intestine

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly increased expression in day 8 adults comparing to in day 1 adults in intestine.

WBPaper00066447:Day8_vs_Day1_upregulated_hypodermis

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly increased expression in day 8 adults comparing to in day 1 adults in hypodermis.

WBPaper00066447:Day8_vs_Day1_upregulated_neuron

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly increased expression in day 8 adults comparing to in day 1 adults in neuron.

WBPaper00066447:Day8_vs_Day1_downregulated_neuron

Differentially expressed miRNAs were identified using DEGseq based on unique molecular identifier (UMI). A minimum UMI sum of 10 in 3 replicates was set as the threshold of expression. MiRNAs with more than five reads were defined as expressed. Differential expression of miRNAs was analysed by t-test (P value < 0.05 and fold-change > 1.5 or < 0.67) after Box-Cox transformation. MiRNA targets were identified by TargetScanWorm (Release 6.2) and Pearson Correlation Coefficient smaller than -0.2. Micro RNAs that showed significantly decreased expression in day 8 adults comparing to in day 1 adults in neuron.