- WBPaper00046415:lithium_downregulated
All data were analyzed using Stat View J 5.0, with all experimental data checked for assumptions of homogeneity of variance across manipulations using Bartletts test. Once the assumptions were satisfied, the data were analyzed by one-way analysis of variance followed by Dunnetts multiple comparison test. When homogeneity was not evident in the data, we used the nonparametric KruskalWallis test, followed by the MannWhitney U-test with a Bonferroni adjustment. Differences were considered significant at P < 0.05.
Genes suppressed following 24h exposure to lithium compounds (78uM LiCl and 375uM Li2CO3), according to custom DNA microarray.
- WBPaper00030839:Larval_A_Class
A two-class unpaired analysis of the data was performed to identify genes that differ by >= 1.5-fold from the reference at a FDR of <1% for the larval pan-neural, embryonic pan-neural, and larval A-class motor neuron datasets.
Larval A-class motor neuron enriched genes.
- WBPaper00030839:Embryo_A_Class
A two-class unpaired analysis of the data was performed to identify genes that differ by >= 1.5-fold from the reference at a FDR of <1% for the larval pan-neural, embryonic pan-neural, and larval A-class motor neuron datasets.
Embryonic A-class motor neuron enriched genes.
- WBPaper00032454:IR_upregulated
To identify differentially expressed genes, gene expression intensity was compared using a moderated t-test and a Bayes smoothing approach developed for a low number of replicates.
Genes up-regulated following ionizing radiation (IR) treatment.
- WBPaper00065746:tmc-1(rg1003)_upregulated
DESeq2. The genes with a fold change >= 2 and a false discovery rate (FDR) < 0.05 in a comparison were identified as significant DEGs.
Transcripts that showed significantly increased expression in tmc-1(rg1003) comparing to in N2.
- WBPaper00036464:S.aureus-RN6390_regulated
The two conditions were then compared in Resolver to determine fold change for each probe set and a p-value, using a modified t test. Genes with a 2-fold or greater fold change and a p-value ,0.01 were considered differentially expressed.
Genes with altered expression after 8 h S. aureus infection.
- WBPaper00065746:eat-2(ad1113)_downregulated
DESeq2. The genes with a fold change >= 2 and a false discovery rate (FDR) < 0.05 in a comparison were identified as significant DEGs.
Transcripts that showed significantly decreased expression in eat-2(ad1113) comparing to in N2.
- WBPaper00065746:tmc-1(rg1003)_downregulated
DESeq2. The genes with a fold change >= 2 and a false discovery rate (FDR) < 0.05 in a comparison were identified as significant DEGs.
Transcripts that showed significantly decreased expression in tmc-1(rg1003) comparing to in N2.
- WBPaper00065746:eat-2(ad1113)_upregulated
DESeq2. The genes with a fold change >= 2 and a false discovery rate (FDR) < 0.05 in a comparison were identified as significant DEGs.
Transcripts that showed significantly increased expression in eat-2(ad1113) comparing to in N2.