- WBPaper00065623:106
CellRanger, DecontX, Monocle3, Louvain algorithm.
Single-cell RNA-Seq cell group 106 expressed in: ASH.
- WBPaper00036383:ash-2_L3_regulated
One-class analysis in significance analysis of microarrays (SAM)38 was performed comparing empty vector to ash-2 RNAi using a 5% false discovery rate (FDR). Resulting SAM lists of genes regulated by ASH-2 were compared for genes that were specifically changed in N2, but not in glp-1(e2141ts) mutant worms. A complete linkage hierarchical clustering was performed using Gene Cluster 3.0. Clustering results were analysed further with Java Treeview40. GOstat41 was used to identify overrepresented gene ontology categories in the list of ASH-2 regulated genes that were dependent on the presence of the germline.
Genes that change expression level at L3 larva stage after ash-2 RNAi.
- WBPaper00036383:ash-2_Day8_regulated
One-class analysis in significance analysis of microarrays (SAM)38 was performed comparing empty vector to ash-2 RNAi using a 5% false discovery rate (FDR). Resulting SAM lists of genes regulated by ASH-2 were compared for genes that were specifically changed in N2, but not in glp-1(e2141ts) mutant worms. A complete linkage hierarchical clustering was performed using Gene Cluster 3.0. Clustering results were analysed further with Java Treeview40. GOstat41 was used to identify overrepresented gene ontology categories in the list of ASH-2 regulated genes that were dependent on the presence of the germline.
Genes that change expression level at day 8 adult stage after ash-2 RNAi.
- WBPaper00061651:ASH_enriched
Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.
Transcripts enriched in ASH according to single cell RNAseq.
- WBPaper00051039:ash-2(RNAi)_regulated_germline
Benjamini-Hochberg, FDR = 0.05
Transcripts that showed significantly altered expression in dissected germline tissue by comparing ash-2(RNAi) with empty vector control injected to N2.
- WBPaper00066232:ash-2(RNAi)_downregulated_WT
Differential mRNA expression using DESeq2. mRNAs with a FDR < 0.05 and fold change > 2 considered differentially expressed.
Transcripts that showed significantly decreased expression in ash-2(RNAi) comparing to in wild type animals injected with vector.
- WBPaper00066232:hsf-1(RNAi)_upregulated_ash-2
Differential mRNA expression using DESeq2. mRNAs with a FDR < 0.05 and fold change > 2 considered differentially expressed.
Transcripts that showed significantly increased expression in hsf-1(RNAi) comparing to in ash-2 animals injected with vector.
- WBPaper00066232:hsf-1(RNAi)_downregulated_ash-2
Differential mRNA expression using DESeq2. mRNAs with a FDR < 0.05 and fold change > 2 considered differentially expressed.
Transcripts that showed significantly decreased expression in hsf-1(RNAi) comparing to in ash-2 animals injected with vector.
- WBPaper00066232:ash-2(RNAi)_upregulated_WT
Differential mRNA expression using DESeq2. mRNAs with a FDR < 0.05 and fold change > 2 considered differentially expressed.
Transcripts that showed significantly increased expression in ash-2(RNAi) comparing to in wild type animals injected with vector.