At the time when the Q descendants are beginning their migrations in early L1 larvae 4-4.5 hours post-hatching, Pspon-1::gfp expression was observed in posterior body wall muscles cells, but not in the Q cells as determined by the Q-cell specific marker rdvIs1 (Pegl-17::mCherry) (Branda and Stern, 2000; Ou et al., 2010). Pspon- 1::gfp expression was in body wall muscle cells adjacent to the Q neuroblasts, with expression extending posteriorly to the tail, but only a short distance anteriorly.
In support of a role of ARX-2 in Q cell corpse clearance, ARX-2::GFP was recruited onto Q cell corpses at 37 ± 9 min (mean ± SD; n = 20) after Q cell birth, and the ARX-2::GFP rings lasted for 19 ± 9 min (n = 20). The corpse was completely degraded 34 ± 18 min (n = 20) after the disappearance of ARX-2::GFP, which suggests that ARX-2 may function during the engulfment of Q cell corpses but may not be involved in the final degradation inside the phagosome.
COR-1::GFP is enriched at the leading edge of the Q cell. Quantitative fluorescence analysis showed that the COR-1::GFP intensity was 1.7-fold as strong in the leading edge of migrating Q cells as in the lagging edge.