The expression pattern of lhIs35[Punc::55::gfp] was similar to previous observations (Zhou and Walthall, 1998) except that VD precursor cells were also labeled. GFP was detected in the 13 VD neurons, 11 AS neurons, VA1 and PDB. Punc-55 activity was highest during L2, and was not detected beyond L4, as reported (Zhou and Walthall, 1998).
The egl-27 reporter is expressed in the Y cell in the 1.5-fold embryonic stage as well as during the L1 larval stage, which precedes the initiation of Y transdifferentiation.