NAB-1::GFP expression is restricted to epithelia and neurons. The earliest expression was observed in the hypodermis of 2-fold-stage early embryos. Immediately prior to hatching, this expression became restricted to the epithelial excretory canal and the nervous system, including the central nervous system and the motoneurons (dorsal and ventral nerve cords. In L3 and L4 larvae, NAB-1::GFP also localized transiently at the membranes of the developing vulva epithelia. Also expressed in distal tip cell (pers. comm. from Wesley Hung 11-17-07.)
The C+C enhancer (pOK17.21) activates frequent expression in all pharyngeal muscles. the C+C enhancer also activates expression in non-muscle cells in the pharynx. Expression induced by the C+C enhancer was observed in all pharyngeal cell types including gland cells, neurons and marginal cells as well as muscles. These results define C as an organ-specific subelement.