Expr13166

the SMA-5::EGFP fluorescence was exclusively detected in the cytoplasm of intestinal cells in strain BJ269. This was somewhat in contrast to the previous observations of Watanabe et al. (Watanabe et al., 2005), who found expression of two sma-5 reporter constructs containing 1.6 kb of the upstream region and almost none or all of the gene-coding region not only in the intestine but also in the excretory cell and to some degree in the hypodermis. In addition, our observations were in disagreement with the distribution of another reporter containing 4.9 kb of the region upstream of exon 1 described by the same group whose expression was noted only in the pharynx and hypodermis.

Expr9115

Expressed in ALA neuron. S. Xu, personal communication.

Expr12635

Pclec-60::GFP expressed in intestine, induced by S. aureus.

Expr12912

UNC-33(S) accumulated at the nerve ring of control animals.

Expr13012

Pcye-1::gfp reporter is only transiently expressed in the VPCs at the lateG1/early S-phase.

Expr10587

Endogenous DVC1 was predominantly expressed during S and G2 phases of the cell cycle. Upon exit from mitosis, DVC1 expression was rapidly downregulated with kinetics similar to that of known APC-Cdh1 targets such as cyclin A, and reaccumulated as cells entered S phase.

Expr14846

To determine in which cell(s) tiar-2 functions, we constructed a GFP-TIAR-2 mini-gene translational reporter and observed broad expression in many cell types, including mechanosensory neurons.

Expr12325

HSP-4::GFP was exclusively localized in the PVD soma, colocalizing with a rough ER marker TRAM, HSP-4's endoplasmic reticulum localization pattern is consistent with the observation that its mammalian homolog BiP is localized in rough endoplasmic reticulum.

Expr1170047

Time-lapse fluorescence microscopy was performed, including DIC for morphology. Gene expression patterns were summarized in 4 manners: Average over time, Average over time and at different positions along the anterior-posterior (AP) axis, a voxelized representation over time, and on individual cells overlaid from a reference coordinate dataset (https://doi.org/10.1016/j.ydbio.2009.06.014). The analysis was done with a pipeline based on the multi-purpose image analysis software Endrov (https://doi.org/10.1038/nmeth.2478), which further is needed to browse the raw recording data. Thumbnail movies were also generated, using maximum Z projection for the 3D fluorescence channel. Raw recordings available in the Endrov OST-file format are available at https://www.ebi.ac.uk/biostudies/studies/S-BIAD191?query=S-BIAD191

Expr1170012

Time-lapse fluorescence microscopy was performed, including DIC for morphology. Gene expression patterns were summarized in 4 manners: Average over time, Average over time and at different positions along the anterior-posterior (AP) axis, a voxelized representation over time, and on individual cells overlaid from a reference coordinate dataset (https://doi.org/10.1016/j.ydbio.2009.06.014). The analysis was done with a pipeline based on the multi-purpose image analysis software Endrov (https://doi.org/10.1038/nmeth.2478), which further is needed to browse the raw recording data. Thumbnail movies were also generated, using maximum Z projection for the 3D fluorescence channel. Raw recordings available in the Endrov OST-file format are available at https://www.ebi.ac.uk/biostudies/studies/S-BIAD191?query=S-BIAD191