sem-4::GFP is expressed throughout the M lineage from the 1-M stage to the 18-M stage, and in the SM sub-lineage from the 2-SM stage to the 16-SM stage, before the cells differentiate. When these cells terminally differentiate,
sem-4::GFP becomes undetectable in the differentiated coelomocytes and vulval muscles, but remains expressed in the differentiated BWMs.