Using an affinity-purified polyclonal HCF-1 antibody in immunostaining assays, prominent HCF-1 staining was observed in the nuclei of most, if not all, somatic and germline cells in wild-type worms.
The let-7 promoter drives GFP expression in AVM and ALM touch neurons in L2-stage and YA animals. Expression of the mature let-7 micro-RNA is almost undetectable from embryonic to L2 stages and becomes apparent at L3.
Type-II thiolase was detectable with anti-(P-44) throughout the life cycle of C. elegans; although the amount relative to the proteins of the whole nematode was larger at the larval stages (L1, L2, L3 and L4) than at the embryo (E), young adult (YA) or egg-lying adult (EA) stages.
A transcriptional GFP expression construct driven by trxr-1 promoter was expressed only in M2 neurons. The expression level of translational TRXR-1::gfp was especially high in intestine, vulva and pharynx.
The lury-1prom::lury-1::Venus translational reporter produced fluorescent signals in coelomocytes, which are macrophage-like scavenger cells that endocytose proteins secreted into the body cavity (Fares and Greenwald, 2001), as well as in the M1 and M2 neurons. This result implies that the LURY-1 preproprotein is sorted into the secretory pathway and that LURY-1 peptides can reach distant cells.
Expression of mpk-1::gfp was observed in the rectal epithelial cells, including F and U, in 100% of wild type L1 (16/16), L2 (19/19), L3 (15/15), L4 (21/21) and adult (16/16)worms.