Expr14011

ASK, ASH, ASI (dim & not so consistent), (AWA dim), vulva muscle?, anal depressor muscle

Expr13376

In WT animals, LIN-28 is highly expressed upon hatching and during the L1 stage, and expression decreases throughout development, so that by the L3 it reduced 10- to 20-fold.

Expr1536

Ubiquitous expression were observed throughout development. Two major structures were highly stained at the larval stage and much less so in adults: the tip of the head and the pharyngeal nerve ring.

Expr7715

Expression is very generalised, but quite weak and so may be constitutive. Expression was strongest in the pharynx, vulva and spermatheca, followed by the rest of the somatic gonad. This pattern was seen in 5 of 7 lines.

Expr1514

The 5.4 kb transcript was most abundant in embryos, less so during L1 to L3 stages, and lowest levels were detected at L4. Intense label was found in adult samples but it was probably due to eggs in the uterus.

Expr67

One nucleus in the anterior part of the pharyngeal metacorpus shows expression. The expression is relatively weak and rare compared to most of the patterns described here, but is reproducible and so is included. The position and singularity of expression suggests the pharyngeal interneuron I3 as the cell of interest.

Expr62

Expression is evident in both the metacorpus and terminal bulb of the adult pharynx. B-gal staining seems to be sub-cellularly localised in the pseudocircular m4 muscle cells in the metacorpus; staining in the terminal bulb is restricted to the posterior of the bulb, and so may mark the location of the m7 muscles.

Expr9566

Expression is evident in both the metacorpus and terminal bulb of the adult pharynx. B-gal staining seems to be sub-cellularly localised in the pseudocircular m4 muscle cells in the metacorpus; staining in the terminal bulb is restricted to the posterior of the bulb, and so may mark the location of the m7 muscles.

Expr7709

Generalised expression is seen, late embryo to adult, although expression is stronger in the intestine and posterior pharynx. The generalised expression definitely includes hypodermis, nerve cells, muscle cells and the excretory cell, as the lines are heavily mosaic and so expression in these cell types could be recognised. Expression in the hypodermis includes the tail spike.

Expr3769

gfp expression was detected in the lateral hypodermal seam and the vulva beginning during the L4 stage, when driven by a wild-type fragment extending 1.0 kb upstream from the miR-48 coding sequence (mir-48::gfp). A single head neuron, RID, also consistently expressed gfp, but did so in a nontemporally regulated fashion and served as an internal control.