NHR-25::GFP was evenly distributed prior to the first division in all VPCs, whereas after the first division the pattern became graded: highest in 1 P6.p daughters, lower in 2 P5.p and P7.p daughters, and lowest in 3 P(3,4,8).px. After the third round of cell divisions NHR-25::GFP expression continued in all 22 P(5-7).pxxx cells and remained high during early vulva morphogenesis until it temporarily disappeared by the ''Christmas tree stage''. In wild type animals, NHR-25::GFP was normally expressed in the anchor cell at the time of the first VPC divisions, and subsequently decreased. NHR-25::GFP is expressed in nuclei of seam cells and
hyp7.