Expr13642

A MEC-19:: GFP translational fusion was found throughout the TRN neurite and also on the plasma membrane and spots within the TRN cell body; its expression overlapped only partially with MEC-4 and MEC-2 (Topalidou and Chalfie 2011) in the proximal neurite and cell body. In the cell body, MEC-19 spots also were found to partially overlap with the Golgi marker AMAN-2::TagRFP.

Expr12767

POML-1 was expressed in only a few neurons. In addition to the six touch receptor neurons (Topalidou and Chalfie, 2011), POML-1 was found in the IL1, AIM, ALN, and BDU neurons. In touch receptor neurons, POML-1 localizes to the endoplasmic reticulum. Rescuing and tagged translational fusions of POML-1 are primarily expressed in the TRN cell body, with weak diffuse expression and puncta in the proximal TRN neurite. Consistent with the ER localization of MEC-6, the protein was absent from the TRN surface.