As previously found (Bounoutas et al., 2009b; Sun et al., 2013), a mec-15::GFP translational reporter was expressed in the TRNs, several head and tail neurons, and ventral cord motor neurons.
As previously reported, pitp-1 is expressed in many neurons. We observed a co-lo- calization of the GFP and mCherry fluorescence in the AQR, PQR, and URX neurons, as well as in the BAG O2-sensing neurons (the BAG neurons act reciprocally to the URX neurons and are activated at low O2 levels), indicating that pitp-1 is co-expressed with glb-5 in O2-sensing neurons.