Expr16540

Animals carrying kpIs106 expressed GVD-1::GFP in various somatic tissues including the somatic gonad, vulva and seam cells. Subcellularly, GVD-1::GFP prominently localized to the nucleus but could be observed in the cytoplasm as well. GVD-1::GFP expression in the somatic gonad started at the L1 stage and persisted until the adult stage; its expression was clearly visible in Z1 and Z4, their progeny and the SGP at the L1, L2 and early L3 stages, respectively. In the vulval lineage, GVD-1::GFP was present in P6.p and its descendants. In late L3 and early L4 larvae, we noticed GVD-1::GFP in the spermathecal/uterine precursors. In adults, GVD-1::GFP could be observed in DTCs, Sh cells and spermatheca. GVD-1::GFP is seen in the developing uterus and vulva at the L3 stage, persisting in the uterus and vulval epithelium (VE) at the L4 stage.