In adult males, the SWM-1::mCherry protein was present, as expected, in cells in which we observed the transcriptional reporter [Expr14579]: vas deferens cuboidal cells, diagonal muscles and body wall muscles. Within cuboidal cells, SWM-1::mCherry was contained within large vesicles that were closely associated with the apical membrane, which are not well-characterized but have been shown to contain seminal fluid (Smith and Stanfield, 2011; Lints and Hall, 2009; Kimble and Hirsh, 1979). In muscle cells, we observed SWM-1::mCherry at low levels that appeared to be concentrated near the cell cortex. Interestingly, in
jn62[
swm-1::mCherry], SWM-1::mCherry was also visible in anterior body wall muscles, rather than being restricted to the posterior region, corresponding to slightly higher levels of rescue and protein expression as compared with the Pswm-1 MosSCI reporter strain.