By using primer sets that distinguish between KIN-20 isoforms that initiate at the 1st AUG and those that initiate at the 2nd AUG of
kin-20, we find that there is ~3 fold more of the B, E and F isoforms than the A, C, and D isoforms at all time points throughout development. By using primer sets that distinguish between the differing 3' isoforms, we find that there is ~3 fold more of the B and C isoforms than the A and E isoforms, and ~10 fold more of the A and E isoforms than the D and F isoforms at all time points throughout development. Thus, the B isoform is the major KIN-20 isoform expressed throughout development. While the D and F isoforms are only weakly expressed, the A, E and C isoforms are all easily detectable throughout development.