tbx-2 transcripts was detected starting at the 4E stage (~102 cell stage, 143 min after the 4-cell stage), and with mid-gastrula expression observed by in situ hybridization (Shin-i and Kohara, http://nematode.lab.nig.ac.jp/).
Endogenous DVC1 was predominantly expressed during S and G2 phases of the cell cycle. Upon exit from mitosis, DVC1 expression was rapidly downregulated with kinetics similar to that of known APC-Cdh1 targets such as cyclin A, and reaccumulated as cells entered S phase.
To determine in which cell(s) tiar-2 functions, we constructed a GFP-TIAR-2 mini-gene translational reporter and observed broad expression in many cell types, including mechanosensory neurons.
HSP-4::GFP was exclusively localized in the PVD soma, colocalizing with a rough ER marker TRAM, HSP-4's endoplasmic reticulum localization pattern is consistent with the observation that its mammalian homolog BiP is localized in rough endoplasmic reticulum.
In early embryonic stages (28-350 cells), cells react with LIN_26 antibodies are ectoblasts. LIN-26 protein continuously present throughout embryonic, larval and adult life in all hypodermal cells, and in all of the glial-like socket and sheath cells associated with ciliated sensory organs. LIN-26 protein was also present in blast cells G1, Q and W. Expression in all three of these cells cease as they divided. Strong expression in Z1 and Z4 in young L1 larvae, weak expression in all cells in L2 larvae except the distal tip cells, and in the uterine cells of L4 larvae and adults.