CLC-2-GFP was detected in hypodermal seam cells, which fuse to form a single elongated syncytium. However, in contrast to CLC-1-GFP, under the expression condition used in this study, GFP signals did not appear to be concentrated at the borders between the seam cell syncytium and surrounding hypodermal cells. When adult wild-type worms were whole-mount stained with anti-CLC-2 pAb, the seam cell syncytium appeared to be outlined to give two parallel lines, although these lines were thick and not very sharp.
The C+C enhancer (pOK17.21) activates frequent expression in all pharyngeal muscles. the C+C enhancer also activates expression in non-muscle cells in the pharynx. Expression induced by the C+C enhancer was observed in all pharyngeal cell types including gland cells, neurons and marginal cells as well as muscles. These results define C as an organ-specific subelement.